Review



chip grade antibody ubiquitin  (Enzo Biochem)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 90
      Buy from Supplier

    Structured Review

    Enzo Biochem chip grade antibody ubiquitin
    CPI-7c efficiently degrades MDM2 but leads to stabilization of p53 and induces global ubiquitination response. In ( A ), MCF-7 cells were treated with 5, 10, 15 and 20 µM CPI-7c and Nutlin-3, and expression levels of MDM2, <t>ubiquitin</t> and p53 were assessed by using immunoblotting and shown in the image. In panel ( B ), MCF-7 cells were grown in coverslips and treated with either vehicle or 10 and 20 µM of CPI-7c and Nutlin-3, respectively, for 24h and subjected to immunofluorescence staining and analyzed by confocal microscope. Merged confocal photographs represent the superimposition of green (p53) and red (MDM2) images, and the magnified area of the box was shown in inset pictures. Scale bar, 20 µm. Representative of three independent experiments. In panel ( C ), RT–PCR analysis shows the mRNA expression MDM2 and GAPDH genes in response to 10 and 20 µM doses of CPI-7c and Nutlin-3 treatment.
    Chip Grade Antibody Ubiquitin, supplied by Enzo Biochem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/chip grade antibody ubiquitin/product/Enzo Biochem
    Average 90 stars, based on 1 article reviews
    chip grade antibody ubiquitin - by Bioz Stars, 2026-05
    90/100 stars

    Images

    1) Product Images from "Dual targeting of MDM2 with a novel small-molecule inhibitor overcomes TRAIL resistance in cancer"

    Article Title: Dual targeting of MDM2 with a novel small-molecule inhibitor overcomes TRAIL resistance in cancer

    Journal: Carcinogenesis

    doi: 10.1093/carcin/bgw088

    CPI-7c efficiently degrades MDM2 but leads to stabilization of p53 and induces global ubiquitination response. In ( A ), MCF-7 cells were treated with 5, 10, 15 and 20 µM CPI-7c and Nutlin-3, and expression levels of MDM2, ubiquitin and p53 were assessed by using immunoblotting and shown in the image. In panel ( B ), MCF-7 cells were grown in coverslips and treated with either vehicle or 10 and 20 µM of CPI-7c and Nutlin-3, respectively, for 24h and subjected to immunofluorescence staining and analyzed by confocal microscope. Merged confocal photographs represent the superimposition of green (p53) and red (MDM2) images, and the magnified area of the box was shown in inset pictures. Scale bar, 20 µm. Representative of three independent experiments. In panel ( C ), RT–PCR analysis shows the mRNA expression MDM2 and GAPDH genes in response to 10 and 20 µM doses of CPI-7c and Nutlin-3 treatment.
    Figure Legend Snippet: CPI-7c efficiently degrades MDM2 but leads to stabilization of p53 and induces global ubiquitination response. In ( A ), MCF-7 cells were treated with 5, 10, 15 and 20 µM CPI-7c and Nutlin-3, and expression levels of MDM2, ubiquitin and p53 were assessed by using immunoblotting and shown in the image. In panel ( B ), MCF-7 cells were grown in coverslips and treated with either vehicle or 10 and 20 µM of CPI-7c and Nutlin-3, respectively, for 24h and subjected to immunofluorescence staining and analyzed by confocal microscope. Merged confocal photographs represent the superimposition of green (p53) and red (MDM2) images, and the magnified area of the box was shown in inset pictures. Scale bar, 20 µm. Representative of three independent experiments. In panel ( C ), RT–PCR analysis shows the mRNA expression MDM2 and GAPDH genes in response to 10 and 20 µM doses of CPI-7c and Nutlin-3 treatment.

    Techniques Used: Expressing, Western Blot, Immunofluorescence, Staining, Microscopy, Reverse Transcription Polymerase Chain Reaction

    CPI-7c physically binds to RING and N-terminal domains of MDM2 and selectively induces ubiquitination of MDM2 as well as interferes at p53–MDM2 interaction. MCF-7 cells were treated with 10 µM dose of CPI-7c and Nutlin-3 for 24h, and protein lysates were prepared for immunoprecipitation using human anti-ubiquitin. Ubiqutinated MDM2 level was measured using western blotting of anti-MDM2 antibody with proper input control and shown in panel ( A ). Similarly, ( B ) represents the level of associated p53 when lysate was immunoprecipitated using anti-MDM2 antibody. Panel ( C ) represents standard scatchard exponential hyperbolic binding curve of direct physical binding of CPI-7c and Nutlin-3 with N-terminal peptide and RING domain peptide of MDM2 in left and right panels, respectively, along with respective dissociation equilibrium constant ( Kd ) values.
    Figure Legend Snippet: CPI-7c physically binds to RING and N-terminal domains of MDM2 and selectively induces ubiquitination of MDM2 as well as interferes at p53–MDM2 interaction. MCF-7 cells were treated with 10 µM dose of CPI-7c and Nutlin-3 for 24h, and protein lysates were prepared for immunoprecipitation using human anti-ubiquitin. Ubiqutinated MDM2 level was measured using western blotting of anti-MDM2 antibody with proper input control and shown in panel ( A ). Similarly, ( B ) represents the level of associated p53 when lysate was immunoprecipitated using anti-MDM2 antibody. Panel ( C ) represents standard scatchard exponential hyperbolic binding curve of direct physical binding of CPI-7c and Nutlin-3 with N-terminal peptide and RING domain peptide of MDM2 in left and right panels, respectively, along with respective dissociation equilibrium constant ( Kd ) values.

    Techniques Used: Immunoprecipitation, Western Blot, Binding Assay



    Similar Products

    chip grade antibody ubiquitin  (Enzo Biochem)
    90
    Enzo Biochem chip grade antibody ubiquitin
    CPI-7c efficiently degrades MDM2 but leads to stabilization of p53 and induces global ubiquitination response. In ( A ), MCF-7 cells were treated with 5, 10, 15 and 20 µM CPI-7c and Nutlin-3, and expression levels of MDM2, <t>ubiquitin</t> and p53 were assessed by using immunoblotting and shown in the image. In panel ( B ), MCF-7 cells were grown in coverslips and treated with either vehicle or 10 and 20 µM of CPI-7c and Nutlin-3, respectively, for 24h and subjected to immunofluorescence staining and analyzed by confocal microscope. Merged confocal photographs represent the superimposition of green (p53) and red (MDM2) images, and the magnified area of the box was shown in inset pictures. Scale bar, 20 µm. Representative of three independent experiments. In panel ( C ), RT–PCR analysis shows the mRNA expression MDM2 and GAPDH genes in response to 10 and 20 µM doses of CPI-7c and Nutlin-3 treatment.
    Chip Grade Antibody Ubiquitin, supplied by Enzo Biochem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/chip grade antibody ubiquitin/product/Enzo Biochem
    Average 90 stars, based on 1 article reviews
    chip grade antibody ubiquitin - by Bioz Stars, 2026-05
    90/100 stars
    		  Buy from Supplier
    chip grade antibodies against ubiquitin-h2a 05-678  (Millipore)
    90
    Millipore chip grade antibodies against ubiquitin-h2a 05-678
    BMI1 directly suppresses Zmym3 and activates c-fos pathway. (A) Electrophoresis strips of Zmym3 promoter region in BMI1 and <t>H2A</t> with ubiquitination ChIP-PCR products. (B) The relative transcript level of Zmym3 in Bmi1-transfected K562 cells. The expression level in K562 was set to 1. (C) The relative transcript expression of c-fos in Bmi1-transfected K562. The expression level in K562 was set to 1. (D) The relative histone H3K27 acetylation (H3K27ac) and H3 acetylation (H3ac) level of c-fos promoter region, n = 3. The histone modification in 10% input DNA was set to 1. IgG was as negative control.
    Chip Grade Antibodies Against Ubiquitin H2a 05 678, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/chip grade antibodies against ubiquitin-h2a 05-678/product/Millipore
    Average 90 stars, based on 1 article reviews
    chip grade antibodies against ubiquitin-h2a 05-678 - by Bioz Stars, 2026-05
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    CPI-7c efficiently degrades MDM2 but leads to stabilization of p53 and induces global ubiquitination response. In ( A ), MCF-7 cells were treated with 5, 10, 15 and 20 µM CPI-7c and Nutlin-3, and expression levels of MDM2, ubiquitin and p53 were assessed by using immunoblotting and shown in the image. In panel ( B ), MCF-7 cells were grown in coverslips and treated with either vehicle or 10 and 20 µM of CPI-7c and Nutlin-3, respectively, for 24h and subjected to immunofluorescence staining and analyzed by confocal microscope. Merged confocal photographs represent the superimposition of green (p53) and red (MDM2) images, and the magnified area of the box was shown in inset pictures. Scale bar, 20 µm. Representative of three independent experiments. In panel ( C ), RT–PCR analysis shows the mRNA expression MDM2 and GAPDH genes in response to 10 and 20 µM doses of CPI-7c and Nutlin-3 treatment.

    Journal: Carcinogenesis

    Article Title: Dual targeting of MDM2 with a novel small-molecule inhibitor overcomes TRAIL resistance in cancer

    doi: 10.1093/carcin/bgw088

    Figure Lengend Snippet: CPI-7c efficiently degrades MDM2 but leads to stabilization of p53 and induces global ubiquitination response. In ( A ), MCF-7 cells were treated with 5, 10, 15 and 20 µM CPI-7c and Nutlin-3, and expression levels of MDM2, ubiquitin and p53 were assessed by using immunoblotting and shown in the image. In panel ( B ), MCF-7 cells were grown in coverslips and treated with either vehicle or 10 and 20 µM of CPI-7c and Nutlin-3, respectively, for 24h and subjected to immunofluorescence staining and analyzed by confocal microscope. Merged confocal photographs represent the superimposition of green (p53) and red (MDM2) images, and the magnified area of the box was shown in inset pictures. Scale bar, 20 µm. Representative of three independent experiments. In panel ( C ), RT–PCR analysis shows the mRNA expression MDM2 and GAPDH genes in response to 10 and 20 µM doses of CPI-7c and Nutlin-3 treatment.

    Article Snippet: Nutlin-3 and ChIP grade antibody for ubiquitin, and HDM2 (catalytic RING domain) peptide were purchased from Enzo Life Sciences.

    Techniques: Expressing, Western Blot, Immunofluorescence, Staining, Microscopy, Reverse Transcription Polymerase Chain Reaction

    CPI-7c physically binds to RING and N-terminal domains of MDM2 and selectively induces ubiquitination of MDM2 as well as interferes at p53–MDM2 interaction. MCF-7 cells were treated with 10 µM dose of CPI-7c and Nutlin-3 for 24h, and protein lysates were prepared for immunoprecipitation using human anti-ubiquitin. Ubiqutinated MDM2 level was measured using western blotting of anti-MDM2 antibody with proper input control and shown in panel ( A ). Similarly, ( B ) represents the level of associated p53 when lysate was immunoprecipitated using anti-MDM2 antibody. Panel ( C ) represents standard scatchard exponential hyperbolic binding curve of direct physical binding of CPI-7c and Nutlin-3 with N-terminal peptide and RING domain peptide of MDM2 in left and right panels, respectively, along with respective dissociation equilibrium constant ( Kd ) values.

    Journal: Carcinogenesis

    Article Title: Dual targeting of MDM2 with a novel small-molecule inhibitor overcomes TRAIL resistance in cancer

    doi: 10.1093/carcin/bgw088

    Figure Lengend Snippet: CPI-7c physically binds to RING and N-terminal domains of MDM2 and selectively induces ubiquitination of MDM2 as well as interferes at p53–MDM2 interaction. MCF-7 cells were treated with 10 µM dose of CPI-7c and Nutlin-3 for 24h, and protein lysates were prepared for immunoprecipitation using human anti-ubiquitin. Ubiqutinated MDM2 level was measured using western blotting of anti-MDM2 antibody with proper input control and shown in panel ( A ). Similarly, ( B ) represents the level of associated p53 when lysate was immunoprecipitated using anti-MDM2 antibody. Panel ( C ) represents standard scatchard exponential hyperbolic binding curve of direct physical binding of CPI-7c and Nutlin-3 with N-terminal peptide and RING domain peptide of MDM2 in left and right panels, respectively, along with respective dissociation equilibrium constant ( Kd ) values.

    Article Snippet: Nutlin-3 and ChIP grade antibody for ubiquitin, and HDM2 (catalytic RING domain) peptide were purchased from Enzo Life Sciences.

    Techniques: Immunoprecipitation, Western Blot, Binding Assay

    BMI1 directly suppresses Zmym3 and activates c-fos pathway. (A) Electrophoresis strips of Zmym3 promoter region in BMI1 and H2A with ubiquitination ChIP-PCR products. (B) The relative transcript level of Zmym3 in Bmi1-transfected K562 cells. The expression level in K562 was set to 1. (C) The relative transcript expression of c-fos in Bmi1-transfected K562. The expression level in K562 was set to 1. (D) The relative histone H3K27 acetylation (H3K27ac) and H3 acetylation (H3ac) level of c-fos promoter region, n = 3. The histone modification in 10% input DNA was set to 1. IgG was as negative control.

    Journal: Journal of Cellular and Molecular Medicine

    Article Title: BMI1 reprogrammes histone acetylation and enhances c-fos pathway via directly binding to Zmym3 in malignant myeloid progression

    doi: 10.1111/jcmm.12246

    Figure Lengend Snippet: BMI1 directly suppresses Zmym3 and activates c-fos pathway. (A) Electrophoresis strips of Zmym3 promoter region in BMI1 and H2A with ubiquitination ChIP-PCR products. (B) The relative transcript level of Zmym3 in Bmi1-transfected K562 cells. The expression level in K562 was set to 1. (C) The relative transcript expression of c-fos in Bmi1-transfected K562. The expression level in K562 was set to 1. (D) The relative histone H3K27 acetylation (H3K27ac) and H3 acetylation (H3ac) level of c-fos promoter region, n = 3. The histone modification in 10% input DNA was set to 1. IgG was as negative control.

    Article Snippet: ChIP grade antibodies against BMI1 (ab14389; Abcam, Cambridge, MA, USA), acetyl-H3 (06-599; Millipore), acetyl-H4 (06-598; Millipore), acetyl-H3K27 (ab4729; Abcam), ubiquitin-H2A (05-678; Millipore), trimethyl-H3K9 (ab8898; Abcam), trimethyl-H3K27 (ab6002; Abcam) and normal Mouse IgG(12-371; Millipore) were used at 2 μg per condition.

    Techniques: Electrophoresis, Ubiquitin Proteomics, Transfection, Expressing, Modification, Negative Control